Rapid analysis of protein expression and solubility with the SpyTag-SpyCatcher system
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- 作者：Dustin Dovala ; William S. Sawyer ; Christopher M. Rath ; Louis E. Metzger IV ; louis.metzger_iv@novartis.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：SpyCatcher ; SpyTag ; Expression test ; Solubility ; Dispersity ; Western
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：44-51
- 全文大小：893 K
文摘
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Fluorescent SpyCatcher can be used to probe Western blots akin to antibodies.
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Fluorescent SpyCatcher enables Western-like detection directly in SDS gels.
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Fluorescent SpyCatcher can be used for F-SEC, measuring dispersity and aggregation.
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SpyCatcher is highly overexpressed and is easily conjugated to fluorophores.