A new method for vitrifying samples for cryoEM

详细信息    查看全文

• 作者：Ivan Razinkov¹ ; Venkata P. Dandey¹ ; Hui Wei ; Zhening Zhang ; David Melnekoff ; William J. Rice ; Christoph Wigge ; Clinton S. Potter ; Bridget Carragher ; ^{bcarr@nysbc.org" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CryoTEM ; Automation ; Self-blotting grids ; Nanowires
• 刊名：Journal of Structural Biology
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：195
• 期：2
• 页码：190-198
• 全文大小：5697 K

文摘

Almost every aspect of cryo electron microscopy (cryoEM) has been automated over the last few decades. One of the challenges that remains to be addressed is the robust and reliable preparation of vitrified specimens of suitable ice thickness. We present results from a new device for preparing vitrified samples. The successful use of the device is coupled to a new “self-blotting” grid that we have developed to provide a method for spreading a sample to a thin film without the use of externally applied filter paper. This new approach has the advantage of using small amounts of protein material, resulting in large areas of ice of a well defined thickness containing evenly distributed single particles. We believe that these methods will in the future result in a system for vitrifying grids that is completely automated.