Countercurrent saccharification of lime-pretreated corn stover - Part 1

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• 作者：Sagar Lonkar ; ^{sklonkar@tamu.edu} ; Chao Liang ; Austin Bond ; Pratik Darvekar ; Heather Brooks ; John Derner ; Russell Yang ; Evan Wilson ; Sebastian Garcia ; M. Nazmul Karim ; Mark Holtzapple
• 关键词：Biofuels ; Countercurrent saccharification ; Pretreated corn stover ; Sugar yield ; Enzyme inhibition
• 刊名：Biomass and Bioenergy
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：28-37
• 全文大小：2620 K
• 卷排序：96

文摘

In batch saccharification at constant cellulase loading, both glucose and xylose yields increased with hemicellulase addition. In batch saccharification, glucose and xylose yield decreased with increasing initial spiked glucose concentration. In countercurrent saccharification, at enzyme loading of 1 mg CTec3/g biomass, glucose, xylose, and total sugar yields were 61%, 41%, and 55%, respectively. In countercurrent saccharification, at enzyme loading of (1 mg CTec3 + 1 mg HTec3)/g biomass, glucose, xylose, and total sugar yields were 67%, 53%, and 62%, respectively. To achieve the same glucan conversion as compared to batch, countercurrent saccharification reduced enzyme loading by 1.6 and 1.4 times at 1 and 2 mg protein/g biomass, respectively.