To determine the role of NKCC1 and WNK1 in spinal cord tissues in the acute and chronic phases following contusional SCI.
An experimental study investigating the phosphorylation profile of an important Cl-regulatory protein Na+-K+-Cl鈭?cotransporter 1 (NKCC1) and its regulatory-kinase WNK1 (kinase with-no-lysine).
Sprague-Dawley rats underwent a contusive SCI at T9. The epicenter spinal cord tissues were harvested at Days 1, 3, and 7 for acute phase of injury or Days 35 and 42 in the chronic phase of injury. Western blot was used to compare phosphorylated levels of both NKCC1 and WNK1 in injured tissues compared with those of sham.
A sustained increase in phosphorylation of NKCC1 and WNK1 was detected in the lesion epicenter in spinal cord during both acute and chronic phases following SCI.
These results suggest that persistent activation of NKCC1 and WNK1 may play an important role in SCI.