Development of TaqMan real-time reverse transcription-polymerase chain reaction for the detection and quantitation of porcine kobuvirus
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- 作者：Xiangdong Zhua ; zhuxd87@126.com" class="auth_mail" title="E-mail the corresponding author ; xiangdongzhu@sina.com" class="auth_mail" title="E-mail the corresponding author ; Yufei Wanga ; b ; wangyufei0204@163.com" class="auth_mail" title="E-mail the corresponding author ; Jianfei Chena ; chenjianfei@126.com" class="auth_mail" title="E-mail the corresponding author ; Xin Zhanga ; zhangxin2410@163.com" class="auth_mail" title="E-mail the corresponding author ; Hongyan Shia ; shy2005y@163.com" class="auth_mail" title="E-mail the corresponding author ; Da Shia ; dashi198566@163.com" class="auth_mail" title="E-mail the corresponding author ; Jing Gaoa ; c ; arisia@126.com" class="auth_mail" title="E-mail the corresponding author ; Li Fenga ; fl@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Porcine kobuvirus (PKV) ; TaqMan real-time RT-PCR ; Conventional RT-PCR
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：234
- 期：Complete
- 页码：132-136
- 全文大小：1060 K
文摘
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Porcine kobuvirus(PKV) is a new emerging virus detected in diarrhea pigs.
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A pair of primers and probe was designed to amplify the conservative 3D region of PKV genome.
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The developed TaqMan probe-based real-time RT-PCR possessed well specificity and had ∼1000 times higher sensitivity than common RT-PCR, and the detection limit was as low as 30 DNA copies.
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The developed TaqMan RT-PCR can be used for early detection and quantitation of PKV.