Development of a real-time PCR procedure for quantification of viable Escherichia coli in populations of E.聽coli exposed to lactic acid, and the acid tolerance of verotoxigenic E.聽coli (VTEC) from cattle hides
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- 作者：Hui Wang ; Colin O. Gill ; Xianqin Yang ; xianqin.yang@agr.gc.ca" class="auth_mail
- 关键词：Escherichia coli ; Verotoxigenic E. coli ; Lactic acid ; Real-time PCR ; Acid tolerance
- 刊名：Food Control
- 出版年：September 2014
- 年：2014
- 卷：43
- 期：Complete
- 页码：104-109
- 全文大小：317 K
文摘
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The cell membranes of Escherichia coli killed by 4% lactic acid (LA) were mostly intact.
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A real-time PCR procedure was developed for enumeration of E. coli surviving LA treatment.
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Cells carrying stx2 and/or eae genes were more acid resistant than E. coli generally.