NMR-based metabonomics study on the effect of Gancao in the attenuation of toxicity in rats induced by Fuzi

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• 作者：Bo Sun^a ; ¹ ; ^{sunbo@proteomics.cn" class="auth_mail" title="E-mail the corresponding author} ; Xubin Wang^a ; ¹ ; ^{wangxubin2012@126.com" class="auth_mail" title="E-mail the corresponding author} ; Ruili Cao^a ; ^{crlmail@163.com" class="auth_mail" title="E-mail the corresponding author} ; Qi Zhang^a ; ^{ncba_nmr@126.com" class="auth_mail" title="E-mail the corresponding author} ; Qiao Liu^a ; ^c ; ^{liuqiao.707@163.com" class="auth_mail" title="E-mail the corresponding author} ; Meifeng Xu^a ; ^{xumeifeng103@126.com" class="auth_mail" title="E-mail the corresponding author} ; Ming Zhang^a ; ^b ; ^{zhangmingln@126.com" class="auth_mail" title="E-mail the corresponding author} ; Xiangbo Du^a ; ^{d5194u@163.com" class="auth_mail" title="E-mail the corresponding author} ; Fangting Dong^a ; ^{dft@proteomics.cn" class="auth_mail" title="E-mail the corresponding author} ; Xianzhong Yan^a ; ^{yanxz@proteomics.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Aconitum carmichaelii Debeaux ; Glycyrrhiza uralensis Fisch. ; Metabonomics ; Toxicity ; NMR ; Chinese herbal medicine
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：617-626
• 全文大小：1262 K

文摘

Fuzi, the processed lateral root of Aconitum carmichaelii Debeaux, is a traditional Chinese medicine used for its analgesic, antipyretic, anti-rheumatoid arthritis and anti-inflammation effects; however, it is also well known for its toxicity. Gancao, the root of Glycyrrhiza uralensis Fisch., is often used concurrently with Fuzi to alleviate its toxicity. However, the mechanism of detoxication is still not well clear.

Aim of the study

In this study, the effect of Gancao on the metabolic changes induced by Fuzi was investigated by NMR-based metabonomic approaches.

Materials and methods

Fifty male Wistar rats were randomly divided into five groups (group A: control, group B: Fuzi decoction alone, group C: Gancao decoction alone, group D: Fuzi decoction and Gancao decoction simultaneously, group E: Fuzi decoction 5 h after Gancao decoction) and urine samples were collected for NMR-based metabolic profiling analysis. Statistical analyses such as unsupervised PCA, t-test, hierarchical cluster, and pathway analysis were used to detect the effects of Gancao on the metabolic changes induced by Fuzi.

Results

The behavioral and biochemical characteristics showed that Fuzi exhibited toxic effects on treated rats (group B) and statistical analyses showed that their metabolic profiles were in contrast to those in groups A and C. However, when Fuzi was administered with Gancao, the metabolic profiles became similar to controls, whereby Gancao reduced the levels of trimethylamine N-oxide, betaine, dimethylglycine, valine, acetoacetate, citrate, fumarate, 2-ketoglutarate and hippurate, and regulated the concentrations of taurine and 3-hydroxybutyrate, resulting in a decrease in toxicity. Furthermore, important pathways that are known to be involved in the effect of Gancao on Fuzi, including phenylalanine, tyrosine and tryptophan biosynthesis, the synthesis and degradation of ketone bodies, and the TCA cycle, were altered in co-treated rats.

Conclusions

Gancao treatment mitigated the metabolic changes altered by Fuzi administration in rats, demonstrating that dosing with Gancao could reduce the toxicity of Fuzi at the metabolic level. Fuzi and Gancao administered simultaneously resulted in improved toxicity reduction than when Gancao was administrated 5 h prior to Fuzi. In summary, co-administration of Gancao with Fuzi reduces toxicity at the metabolic level.