Overexpression of a novel NAC-type tomato transcription factor, SlNAM1, enhances the chilling stress tolerance of transgenic tobacco

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• 作者：Xiao-Dong Li¹ ; Kun-Yang Zhuang¹ ; Zhong-Ming Liu ; Dong-Yue Yang ; Na-Na Ma ; ^{nnma@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Qing-Wei Meng ; ^{qwmeng@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ABA ; abscisic acid ; ABRE ; ABA-responsive elements ; APX ; ascorbate peroxidase ; CaMV 35S ; cauliflower mosaic virus 35S ; CBF ; C-repeat binding factor ; DAB ; 3 ; 3⿲ -diaminobenzidine ; DRE ; dehydration responsive element ; DREB ; drought responsive element binding proteins ; GFP ; green fluorescent protein ; H2O2 ; hydrogen peroxide ; ICE ; inducer of CBF expression ; JA ; jasmonic acid ; LEA ; late embryogenesis abundant proteins ; LTRE ; low temperature responsive element ; MDA ; malondialdehyde ; MeJA ; Methyl Jasmona
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 October 2016
• 年：2016
• 卷：204
• 期：Complete
• 页码：54-65
• 全文大小：3371 K

文摘

The NAC proteins are the largest transcription factors in plants. The functions of NACs are various and we focus on their roles in response to abiotic stress here. In our study, a typical NAC gene (SlNAM1) is isolated from tomato and its product is located in the nucleus. It also has a transcriptional activity region situated in C-terminal. The expression levels of SlNAM1 in tomato were induced by 4 °C, PEG, NaCl, abscisic acid (ABA) and methyl jasmonate (MeJA) treatments. The function of SlNAM1 in response to chilling stress has been investigated. SlNAM1 overexpression in tobacco exhibited higher germination rates, minor wilting, and higher photosynthetic rates (Pn) under chilling stress. Meanwhile, overexpression of SlNAM1 improved the osmolytes contents and reduced the H₂O₂ and O₂^⿢⿿ contents under low temperature, which contribute to alleviating the oxidative damage of cell membrane after chilling stress. Moreover, the transcripts of NtDREB1, NtP5CS, and NtERD10s were higher in transgenic tobacco, and those increased expressions may confer higher chilling tolerance of transgenic plants. These results indicated that overexpression of SlNAM1 could improve chilling stress tolerance of transgenic tobacco.