Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats

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• 作者：Ying Zhou¹ ; Huai-rui Yuan¹ ; Lu Cui ; Abdur Rahman Ansari ; Ke Xiao ; You Luo ; Xin-tong Wu ; Liang Guo ; Faheem Ahmed Khan ; Zhi Yang ; Hui Song ; ^{songh2007@mail.hzau.edu.cn}
• 关键词：Visfatin ; LPS induced ; Intestine mucosal ; Apoptosis ; Caspase-3
• 刊名：Acta Histochemica
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：119
• 期：1
• 页码：26-31
• 全文大小：3091 K
• 卷排序：119

文摘

This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n = 10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa. Similarly, ELISA and western blot analysis also showed the up-regulation of pro-caspase-3 and cleaved caspase-3 expression in the visfatin group compared to the control group. In contrast to LPS, visfatin down-regulated the expression of cleaved-caspase-3 in the visfatin+LPS co-stimulated group, resulting in a significant decrease in apoptosis in intestinal mucosal cells. We observed more pro-caspase-3 positive cells in the visfatin+LPS co-stimulated group. The results indicate that, in the presence of LPS, visfatin plays an important role in the regulation of cell apoptosis and inflammation.