Production of Escherichia coli-based virus-like particle vaccine against porcine circovirus type 2 challenge in piglets: Structure characterization and protective efficacy validation
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- 作者：Xiangfeng Xia ; 1 ; Xiaobing Moc ; d ; 1 ; Yan Xiaoa ; Bo Yinc ; Chaochao Lva ; Yuzhou Wanga ; Zhe Suna ; Qingyuan Yanga ; Yali Yaoa ; Yajie Xuana ; Xiangdong Lia ; Y. Adam Yuanc ; d ; dbsyya@nusri.cn" class="auth_mail" title="E-mail the corresponding author ; Kegong Tiana ; b ; tiankg@263.net" class="auth_mail" title="E-mail the corresponding author
- 关键词：Porcine circovirus type 2 ; Escherichia coli expression ; Virus-like particle ; Cryo-EM structure ; Vaccine efficacy
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 April 2016
- 年：2016
- 卷：223
- 期：Complete
- 页码：8-12
- 全文大小：1814 K
文摘
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Soluble non-tag full-length PCV2 capsid protein (rCap) was produced in Escherichia coli and self-assembled into VLP in vitro.
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The Cryo-EM structure of PCV2 rCap VLP was determined to 4.5 Å resolution.
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PCV2 rCap VLP-based vaccine displayed strong protective efficacy against PCV2 challenge as a commercial vaccine.
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