On-line comprehensive two-dimensional HepG2 cell membrane chromatographic analysis system for charactering anti-hepatoma components from rat serum after oral administration of Radix scutellariae: A strategy for rapid screening active compounds in vivo
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- 作者：Dan Jiaa ; 1 ; Xiaofei Chena ; 1 ; Yan Caoa ; 1 ; Xunxun Wub ; Xuan Dinga ; Hai Zhangc ; Chuan Zhanga ; Yifeng Chaia ; yfchai@smmu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Zhenyu Zhua ; zzyzyfzhu@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Drug-containing serum ; Comprehensive two-dimensional HepG2/cell membrane chromatography system ; Matrix interference elimination strategy ; Anti-hepatoma components ; Herbal medicine
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：25 January 2016
- 年：2016
- 卷：118
- 期：Complete
- 页码：27-33
- 全文大小：1809 K
文摘
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A comprehensive 2D CMC system was developed and applied to characterize active components from drug-containing serum for the first time.
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A matrix interference deduction method effectively eliminated the interference of endogenous substances in serum.
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Wogonin and oroxylin A screened both in vitro and in vivo, could be selected as anti-hepatoma leading compounds.
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The methods had advantages in simplicity, sensitivity, accurate recognition and identification.