Antioxidant activity of a water-soluble polysaccharide purified from Pteridium aquilinum

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• 作者：Wentao Xu ; Fangfang Zhang ; YunBo Luo ; Liyan Ma ; Xiaohong Kou ; Kunlun Huang
• 关键词：Pteridium aquilinum ; Polysaccharide ; Purification ; Antioxidant
• 刊名：Carbohydrate Research
• 出版年：2009
• 出版时间：26 January 2009
• 年：2009
• 卷：344
• 期：2
• 页码：217-222
• 全文大小：427 K

文摘

A water-soluble crude polysaccharide, obtained from fern Pteridium aquilinum, was fractionated by DEAE–Sepharose Fast-Flow column chromatography, and purified by Sephacryl S-400 HR column chromatography. The average molecular weight (M_w) of the purified polysaccharide (PLP) is 458,000 Da. The monosaccharide components of PLP were characterized by gas chromatography (GC), and the majority of the monosaccharide components was glucose (relative mass 58.1 % ) with low levels of galactose, mannose, rhamnose, and arabinose (relative mass 18.7 % , 6.8 % , 10.2 % , and 6.1 % , respectively). The Fourier-transform infrared spectra (FTIR) of PLP revealed typical characteristics of polysaccharides. On the basis of the ferric-reducing antioxidant power assay (FRAP), DPPH radical-scavenging, the superoxide radical assay, and self-oxidation of 1,2,3-phentriol assay, the antioxidant activities of PLP were investigated. The purified polysaccharide was demonstrated to have strong reductive power (FRAP value: 827.6 μmol/L), moderate scavenging activities against DPPH radicals (83.1 % ) and superoxide radicals (60.5 % ), and moderate inhibiting power for self-oxidation of 1,2,3-phentriol (52.4 % ).