Expression of bioactive recombinant human fibroblast growth factor 9 in oil bodies of Arabidopsis thaliana
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- 作者：Shanyong Yia ; b ; 1 ; Jing Yanga ; 1 ; Jian Huanga ; b ; Lili Guana ; Linna Dua ; Yongxin Guoa ; Feng Zhaia ; Yanfang Wanga ; Zhen Lua ; Liyong Wanga ; Haiyan Lia ; Xiaokun Lia ; c ; xiaokunli@163.net" class="auth_mail" title="E-mail the corresponding author ; Chao Jianga ; c ; changjiang10@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：rhFGF9 ; recombinant human fibroblast growth factor 9 ; pOTB ; p1301-promoter-oleosin&ndash ; terminator-35S-bar-Nos ; PBS ; sodium phosphate buffer ; WT ; wild Arabidopsis thaliana seeds ; 6-BA ; 6-benzylaminopurine
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：116
- 期：Complete
- 页码：127-132
- 全文大小：862 K
文摘
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The rhFGF9 protein was expressed in plants for the first time.
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The oleosin-fusion technology was first used to express rhFGF9 protein.
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The oil bodies that expressed oleosin–rhFGF9 can stimulate proliferation of NIH/3T3 cells.
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