Pokemon reduces Bcl? expression through NF¡ª?Bp65: a possible mechanism of hepatocellular carcinoma

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• 作者：Xinkai Zhao^a ; ^b ; Qiaoming Ning^b ; Xiaoning Sun^b ; De'an Tian^a ; ^{xinkaizhao@163.com}
• 关键词：Pokemon ; NF-¦Ê B p65 ; Bcl-2 ; Hepatocellular carcinoma
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2011
• 出版时间：June 2011
• 年：2011
• 卷：4
• 期：6
• 页码：492-497
• 全文大小：380 K

文摘

Objective

To investigate the relationship among Pokemon, NF-¦Ê B p65 and Bcl-2 in hepatoma cells.

Methods

HCC cell HepG2, SMMC7721 and human fetal liver cell line LO2 cells were used, and expression of Pokemon, NF-¦Ê B p65 and Bcl-2 in three cells were detected by real-time PCR and western blot. Then siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-¦Ê B p65 and apoptotic rate was determined by flow cytometric analysis.

Results

Expressions of Pokemon, NF-¦Ê B p65 and Bcl-2 in human hepatoma cell HepG2, SMMC7721 expression were significantly higher than those in human embryonic stem cells LO2. siRNA of Pokemon inhibited the expression of Pokemon, NF-¦Ê B p65 and Bcl-2 in liver cancer cells, and significantly increased apoptosis of liver cells. While siRNA of NF-¦Ê B p65 inhibited the expression of NF-¦Ê B p65 and Bcl-2, but Pokemon expression in hepatoma cells had no significant change.

Conclusions

The proto-oncogene Pokemon can inhibit P14ARF by specific transcription regulation of cell cycle and can induce tumors. In addition, Pokemon can regulate NF-¦Ê B p65 through the expression of apoptosis repressor, and promote the development of liver cancer. It suggests signal network in the liver include the regulation of new non-classical NF-¦Ê B regulatory pathway.