A one-step duplex rRT-PCR assay for the simultaneous detection of duck hepatitis A virus genotypes 1 and 3
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- 作者：Qin Hua ; b ; c ; 15283521606@163.com" class="auth_mail" title="E-mail the corresponding author ; Dekang Zhub ; c ; 1 ; zdk24@163.com" class="auth_mail" title="E-mail the corresponding author ; Guangpeng Mad ; 1 ; maguangpeng@163.com" class="auth_mail" title="E-mail the corresponding author ; Anchun Chenga ; b ; c ; chenganchun@vip.163.com" class="auth_mail" title="E-mail the corresponding author ; Mingshu Wanga ; b ; c ; mshwang@163.com" class="auth_mail" title="E-mail the corresponding author ; Shun Chena ; b ; c ; sophia_cs@163.com" class="auth_mail" title="E-mail the corresponding author ; Renyong Jiaa ; b ; c ; cqrc_jry@163.com" class="auth_mail" title="E-mail the corresponding author ; Mafeng Liua ; b ; c ; liumafengra@163.com" class="auth_mail" title="E-mail the corresponding author ; Kunfeng Suna ; b ; c ; sunkunfeng1981@163.com" class="auth_mail" title="E-mail the corresponding author ; Qiao Yanga ; b ; c ; yangqiao721521@sina.com" class="auth_mail" title="E-mail the corresponding author ; Ying Wua ; b ; c ; yingzi_no1@126.com" class="auth_mail" title="E-mail the corresponding author ; Xiaoyue Chenb ; c ; chenxy_24@sina.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Duck hepatitis A virus ; DHAV-1 ; DHAV-3 ; Duplex rRT-PCR
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：207-214
- 全文大小：1346 K
文摘
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The method was sensitive, specific and time-saving that can be used as a reliable and effective tool for the diagnosis, detection and molecular epidemiology investigation of DHAV-1, DHAV-3 and their mixed infection.
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The method of one-step duplex rRT-PCR can simultaneously detect DHAV-1 and DHAV-3 in the same tube.
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The method can help provide timely and effective prevention and control of breeding reservoirs for DHAV.