Kr¨¹ppel-like factor 12 negatively regulates human endometrial stromal cell decidualization

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• 作者：Xiaoyue Shen^a ; ¹ ; Yali Hu^a ; ¹ ; Yue Jiang^a ; Hongyu Liu^a ; Lihua Zhu^a ; Xiaoyan Jin^a ; Huizhi Shan^a ; Xin Zhen^a ; Lihua Sun^b ; Guijun Yan^a ; ^{yanguijun55@163.com} ; Haixiang Sun^a ; ^{stevensun_gl@yahoo.com.cn}
• 关键词：HESCs ; human endometrial stromal cells ; CEBP/¦Â ; CCAAT/enhancer-binding protein-b ; FOXO1A ; forkhead box O1A ; HOXA10 ; homeobox A10 ; ETS1 ; v-ets erythroblastosis virus E26 oncogene homolog 1 ; PRL ; prolactin ; IGFBP-1 ; insulin-like growth factor binding prote
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：29 March, 2013
• 年：2013
• 卷：433
• 期：1
• 页码：11-17
• 全文大小：840 K

文摘

Members of the KLFs family of transcription factors play roles in maternal endometrium development during embryo implantation. However, the specific role of KLF12 in endometrium development has not yet been described. In this study, we showed that KLF12 expression in human endometrial stromal cells (HESCs) was significantly decreased after decidualization stimulated by 8-Br-cAMP and medroxyprogesterone acetate (MPA). The adenovirus-mediated overexpression of KLF12 in HESCs significantly repressed the expression and secretion of decidualization biomarker genes and their products decidual prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1) induced by 8-Br-cAMP and MPA. Moreover, CHIP and luciferase reporter assays demonstrated that KLF12 bound to a CAGTGGG element within the decidual prolactin promoter and decreased decidual PRL promoter (dPRL/-2000Luc) activation in a sequence-specific manner. Taken together, these findings suggest KLF12 is a negative regulator of human endometrial stromal cell decidualization.