Analysis of stereochemistry and biosynthesis of epicatechin in tea plants by chiral phase high performance liquid chromatography
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- 作者：Yumei Qiana ; b ; Xianqian Zhaoc ; Lei Zhaod ; Lilan Cuia ; Li Liua ; Xiaolan Jianga ; Yajun Liuc ; Liping Gaoc ; gaolp62@126.com" class="auth_mail" title="E-mail the corresponding author ; Tao Xiaa ; xiatao62@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：High performance liquid chromatography ; Chiral phase ; Stereochemistry ; Biosynthesis ; Epicatechin ; Tea
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：1006
- 期：Complete
- 页码：1-7
- 全文大小：940 K
文摘
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(−)-EC was detected in tea plants by using chiral phase HPLC.
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(−)-EC was the only product from cyanidin by partially purified native CsANR.
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(+)-EC was main product resulted from cyanidin by recombinant CsANR.
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(−)-EC could result from (+)-C by recombinant CsANR.
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(−)-EC was the product resulted from ECG by partially purified native CsGCH.