A one-pot strategy for the sensitive detection of miRNA by catalyst-oligomer-mediated enzymatic amplification-based fluorescence biosensor
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- 作者：Kai Zhang ; zhangkai@jsinm.org" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Ke WangAuthor Vitae ; Xue ZhuAuthor Vitae ; Minhao XieAuthor Vitae
- 关键词：MiRNA detection ; One-pot ; Nicking endonucleases ; Cell extracts ; Catalyst&ndash ; oligomer-mediated enzymatic amplification
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：223
- 期：Complete
- 页码：586-590
- 全文大小：1299 K
文摘
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MiRNAs is an ideal class of biomarker candidates for cancer classification.
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A one-pot method based on CMEA for the assay of miRNA was proposed.
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The detection limit of the strategy can be lowered to 10 fM of Let-7a.
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The strategy can be used for the detection of Let-7a in cell lysate.