Effects of cerous nitrate on growth and tanshinone production in salvia miltiorrhiza hairy roots

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• 作者：Mingyu HAN ((韩名宇))^a ; ^b ; Wanli GUO ((郭万里))^a ; ^b ; Zongsuo LIANG ((梁宗锁))^a ; ^b ; ^{liangzs@ms.iswc.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Dongfeng YANG ((杨东风))^a ; ^b ; ^{ydf807@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Xijun YAN ((闫希军))^c ; Yonghong ZHU ((朱永宏))^c ; Yan LIU ((刘 岩))^c
• 关键词：tanshinones ; Salvia miltiorrhiza ; Ce3+ ; secondary metabolism ; rare earth element
• 刊名：Journal of Rare Earths
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：33
• 期：11
• 页码：1228-1235
• 全文大小：1227 K

文摘

Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce³⁺ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I (DTI) and cryptotanshinone (CT) were significantly enhanced by 50 μmol/L Ce³⁺, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A (TIIA) and tanshinone I (TI) contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes (HMGR, DXR, DXS1, DXS2 and GGPPS) involved in tanshinone biosynthesis were upregulated by Ce³⁺. Responses of DXS1, DXS2 and GGPPS to Ce³⁺ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.