Dynamically-enhanced retention of gold nanoclusters in HeLa cells following X-rays exposure: A cell cycle phase-dependent targeting approach

详细信息    查看全文

• 作者：Yan Liu^a ; ^b ; ^c ; ^d ; Weiqiang Chen^a ; ^b ; ^c ; Pengcheng Zhang^a ; ^b ; ^c ; ^d ; Xiaodong Jin^a ; ^b ; ^c ; Xinguo Liu^a ; ^b ; ^c ; Ping Li^a ; ^b ; ^c ; Feifei Li^a ; ^b ; ^c ; ^d ; Hongpeng Zhang^a ; Guozhang Zou^e ; ^f ; Qiang Li^a ; ^b ; ^c ; ^{liqiang@impcas.ac.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ionizing radiation ; Nano-agents ; Cell cycle ; Cellular uptake ; Synergistic chemo-radiotherapy
• 刊名：Radiotherapy and Oncology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：119
• 期：3
• 页码：544-551
• 全文大小：1744 K

文摘

Cell cycle phase could affect the cellular uptake of nanoparticles. Based on the fact that ionizing radiation exposure can delay cell cycle progression including inducing G2/M phase arrest, we propose that ionizing radiation exposure is a cell cycle phase-dependent targeting approach for intracellular delivery of nano-agents in tumor cells.

Materials and methods

We synthesized luminescent gold nanoclusters (AuNCs) using a one-pot green synthetic method. Subsequently, we used the as-prepared AuNCs as both “nano-agents” and fluorescent trafficking probes for our study using human cervical carcinoma HeLa cells. Estimating the cellular uptake of AuNCs and cell cycle analysis were performed following X-rays irradiation and cell synchronization.

Results

Our work showed that X-rays irradiation could delay the division of HeLa cells and thereby enhance the retention of AuNCs in HeLa cells, which is a reverse strategy compared with other studies on synergistic nano-radiotherapy. Our results demonstrated that the cell cycle synchronization influenced the cellular uptake processes of AuNCs, suggesting that dynamic cell cycle progression could affect the cellular uptake kinetics of AuNCs.

Conclusion

We consider that the radiation-induced cell division delay might provide a possible mechanism underlying the enhanced effect for the cellular uptake of AuNCs in irradiated HeLa cells.