Molecular cloning, characterization and expression analysis of three heat shock responsive genes from Haliotis diversicolor

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• 作者：Yitao Huang ; Xiuhong Cai ; Zhihua Zou ; Shuhong Wang ; Guodong Wang ; Yilei Wang ; Ziping Zhang
• 关键词：Haliotis diversicolor ; HSF1 ; HSBP1 ; HSP90 ; Stress
• 刊名：Fish & Shellfish Immunology
• 出版年：February, 2014
• 年：2014
• 卷：36
• 期：2
• 页码：590-599
• 全文大小：4050 K

文摘

In this study, molecular characterization and expression of three heat shock responsive genes were analyzed as indicators to understand the mechanism of heat shock response of small abalone Haliotis diversicolor under stresses. The full length cDNA of heat shock transcriptional factor 1 (HdHSF1), heat shock factor binding protein 1(HSBP1), and heat shock protein 90 (HdHSP90) are 1548聽bp, 809聽bp, and 2592聽bp respectively, encoding a protein of 515聽aa, 75聽aa, and 728聽aa respectively. Real time quantitative PCR analysis revealed that these three genes are constitutively expressed in 7 selected tissues. The expression level of HdHSF1 in gills was higher than that in other tissues (p聽<聽0.05). The highest expression level of HdHSBP1 was detected in hemocytes. The highest expression level of HdHSP90 was in the digestive tract and colleterial gland. The HdHSF1 expression level in the gills was up-regulated significantly (p聽<聽0.05) after thermal stress and hypoxia exposure respectively. On the contrary, HdHSBP1 was down-regulated both in gills and hemocytes after thermal stress and the same as in gills after hypoxia stress. HdHSP90 expression level was also up-regulated in gills and hemocytes after both thermal and hypoxia stresses. These results indicated that these three heat shock responsive genes play important roles in response to thermal and hypoxia stress.