Disruption of ku70 involved in non-homologous end-joining facilitates homologous recombination but increases temperature sensitivity in the phytopathogenic fungus Penicillium digitatum
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- 作者：Mó ; nica Gandí ; a ; mgandia@iata.csic.es" class="auth_mail" title="E-mail the corresponding author ; Shaomei Xu Shao_mei93@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Cristina Font cris_font_ma@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Jose F. Marcos jmarcos@iata.csic.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：Citrus fruit ; Gene targeting ; Chitin synthase ; Fungal transformation
- 刊名：Fungal Biology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：120
- 期：3
- 页码：317-323
- 全文大小：712 K
文摘
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Disruption of ku70 enhanced homologous recombination frequency in Penicillium digitatum.
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The mutation of ku70 did not affect P. digitatum phenotypes at optimal temperature.
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Δku70 strains showed defects in growth and conidia production at high temperatures.
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Fungal Δku70 recipient strains should be used with caution.