Immobilized cellulase by polyvinyl alcohol/Fe₂O₃ magnetic nanoparticle to degrade microcrystalline cellulose

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• 作者：Hongdong Liao ; Ding Chen ; Li Yuan ; Mang Zheng ; Yonghua Zhu ; Xuanming Liu
• 关键词：Cellulase immobilization ; Polyvinyl alcohol/Fe₂O₃ nanoparticle ; Microcrystalline cellulose ; Degradation ; Ball milling
• 刊名：Carbohydrate Polymers
• 出版年：2010
• 出版时间：15 October 2010
• 年：2010
• 卷：82
• 期：3
• 页码：600-604
• 全文大小：432 K

文摘

In an attempt to improve the enzymatic efficiency in ball mill, a novel immobilized cellulase on polyvinyl alcohol/Fe₂O₃ magnetic nanoparticle with high activity was synthesized and characterized by transmission electron microscopy, Zetasizer, Fourier transform infrared (FTIR) spectroscopy and vibrating sample magnetometry. It was found that the immobilized cellulase was a kind of spherical complex with approximate 270 nm, 4.87 A m² kg⁻¹, and mainly constructed by the accumulation of 10 nm Fe₂O₃ nanoparticles with the diffused polymer. When the immobilized cellulase was applied to degrade microcrystalline cellulose by combining with wet ball milling, the yielded glucose was 1.89 mg mL⁻¹, at least three times than the sum of individual yield. The immobilized cellulose maintained 40 % activity even after four cycles of reuse. From these results, it can be concluded that the immobilization of cellulase with wet ball milling is a novel method to significantly improve the efficiency of cellulose conversion.