The expression and localization of a novel protein phosphatase inhibitor 2810408A11Rik in mouse testis and sperm

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• 作者：Ye Bi ; Mingxi Liu ; Wenjiao Tu ; Yibo Wu ; Xuejiang Guo ; Zuomin Zhou ; Jiahao Sha ; ^{shajh@njmu.edu.cn}
• 关键词：2810408A11Rik ; testis specific ; protein phosphatase inhibitor ; capacitation
• 刊名：Journal of Biomedical Research
• 出版年：2012
• 出版时间：March, 2012
• 年：2012
• 卷：26
• 期：2
• 页码：110-116
• 全文大小：580 K

文摘

This study investigated the expression and distribution of 2810408A11Rik in mouse testis and sperm, and explored its role in spermatogenesis and sperm function. The expression levels of 2810408A11Rik mRNA in multiple tissue samples were analyzed using bioinformatic resources and RT-PCR technique. A specific rabbit polyclonal antibody was prepared by prokaryotic expression of 2810408A11Rik recombinant protein and utilized for animal immunization. Western blotting, immunohistochemistry and immunofluorescence were used to detect the expression and distribution of 2810408A11Rik. The results of the bioinformatic analysis and RT-PCR showed that 2810408A11Rik mRNA was specifically expressed in mouse testis, and 2810408A11Rik protein included a protein phosphatase inhibitor domain. Western blotting assays, immunohistochemistry and immunofluorescence confirmed the expression of 2810408A11Rik protein in mouse testis, especially in post-meiosis round and long spermatids, and that it is localized in the acrosome and the post-nucleus area of sperm. Our findings suggest that 2810408A11Rik may play an important role in spermatogenesis, sperm capacitation and fertilization.