A new immunoreactive recombinant protein designated as rBoSA2 from Babesia ovis: Its molecular characterization, subcellular localization and antibody recognition by infected sheep
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- 作者：Ferda Sevinca ; fsevinc@selcuk.edu.tr" class="auth_mail" title="E-mail the corresponding author ; Shinuo Caob ; Mo Zhoub ; Mutlu Sevincc ; Onur Ceylana ; Xuenan Xuanb
- 关键词：Babesia ovis ; cDNA library ; Immunoscreening ; rBoSA2 ; ELISA
- 刊名：Veterinary Parasitology
- 出版年：2015
- 出版时间：30 November 2015
- 年：2015
- 卷：214
- 期：1-2
- 页码：213-218
- 全文大小：684 K
文摘
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A complementary DNA expression library was screened to identify immunoreactive proteins of Babesia ovis.
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The cDNA clone designated as BoSA2 was identified by immunoscreening of a cDNA library. The sequence of the BoSA2 was inserted into the expression vector and then expressed in Escherichia coli as a glutathione S-transferase fusion protein.
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Immunoreactivity of the purified recombinant fusion protein (rBoSA2) was evaluated by indirect enzyme-linked immunosorbent assay.
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The results demonstrated that the rBoSA2 protein might be useful for serological study for Babesia ovis infection in sheep.