Electrospun nanofibrous scaffolds of poly (l-lactic acid)-dicalcium silicate composite via ultrasonic-aging technique for bone regeneration

详细信息    查看全文

• 作者：Shengjie Dong ; Junying Sun ; Yadong Li ; Jun Li ; Wenguo Cui ; Bin Li
• 关键词：Dicalcium silicate ; Electrospinning ; Biomineralization ; Cell proliferation ; Differentiation
• 刊名：Materials Science and Engineering: C
• 出版年：1 February, 2014
• 年：2014
• 卷：35
• 期：Complete
• 页码：426-433
• 全文大小：1754 K

文摘

Polymeric nanofibrous composite scaffolds incorporating bioglass and bioceramics have been increasingly promising for bone tissue engineering. In the present study, electrospun poly (l-lactic acid) (PLLA) scaffolds containing dicalcium silicate (C₂S) nanoparticles (approximately 300 nm) were fabricated. Using a novel ultrasonic dispersion and aging method, uniform C₂S nanoparticles were prepared and they were homogenously distributed in the PLLA nanofibers upon electrospinning. In vitro, the PLLA-C₂S fibers induced the formation of HAp on the surface when immersed in simulated body fluid (SBF). During culture, the osteoblastic MC3T3-E1 cells adhered well on PLLA-C₂S scaffolds, as evidenced by the well-defined actin stress fibers and well-spreading morphology. Further, compared to pure PLLA scaffolds without C₂S, PLLA-C₂S scaffolds markedly promoted the proliferation of MC3T3-E1 cells as well as their osteogenic differentiation, which was characterized by the enhanced alkaline phosphatase (ALP) activity. Together, findings from this study clearly demonstrated that PLLA-C₂S composite scaffold may function as an ideal candidate for bone tissue engineering.