Synthesis of octyl-β-d-glucopyranoside catalyzed by Thai rosewood β-glucosidase-displaying Pichia pastoris in an aqueous/organic two-phase system
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- 作者：DongHeng Guo ; YanShan Xu ; YaJun Kang ; ShuangYan Han ; syhan@scut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; SuiPing Zheng ; spzheng@scut.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Thai rosewood β-glucosidase ; Yeast whole-cell catalyst ; Transglycosylation ; Octyl-β-d-glucopyranoside ; Scale synthesis
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：85
- 期：Complete
- 页码：90-97
- 全文大小：1278 K
文摘
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Thai rosewood β-glucosidase yeast whole cells were used to synthesize octyl-glu.
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The efficiency of transglycosylation is much higher than reverse hydrolysis.
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Butyl-glu was used as the substrate in transglycosylation reaction.
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51.1% and 47.5% conversion rates were achieved in 2 ml and 500 ml reaction systems.
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The reuse experiments showed Pp-DCBGL had a good operating stability.