Isolation and characterization of peptidoglycan recognition protein 1 from antler base of sika deer (Cervus nippon)
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- 作者：Wei Jiang ; Yongguang Yin ; Yajun Zhou ; Guidan He ; Yue Qi
- 关键词：Antler base ; Peptidoglycan recognition protein 1 ; MALDI-TOF-MS ; Antimicrobial activity
- 刊名：International Journal of Biological Macromolecules
- 出版年：March, 2014
- 年：2014
- 卷：64
- 期：Complete
- 页码：313-318
- 全文大小：1157 K
文摘
Peptidoglycan recognition proteins (PGRPs) are secreted innate immunity pattern recognition molecules. In this study, a new peptidoglycan recognition protein 1 named cnPGRP1 was isolated from an antler base of sika deer Cervus nippon. The antler base antimicrobial proteins (AAP) were subjected to consecutive chromatographic methods connected to Sephadex G-25 gel filtration column (CM) anion-exchange column, and RP-HPLC. The molecular weight of cnPGRP1 was 17.2 kDa under SDS-PAGE, and peptide mass fingerprint analysis by MALDI-TOF-MS as peptidoglycan recognition protein 1 matched to Dasypus novemcinctus. The matched amino acids sequences were RLYEIIQKWPHYRA. Both Gram-positive and Gram-negative bacteria can be killed by cnPGRP1 in the 50-250 渭g/mL range through in vitro. Furthermore, cnPGRP1 has been found to bind Gram-positive bacteria, Gram-negative bacteria, and even fungus.