High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China

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• 作者：Ji-long HAN^a ; ^{hanjilong10000@126.com" class="auth_mail" title="E-mail the corresponding author} ; Min YANG^b ; Ting-ting GUO^a ; Jian-bin LIU^a ; Chun-e NIU^a ; Chao YUAN^a ; Yao-jing YUE^a ; ^{yueyaojing@126.com" class="auth_mail" title="E-mail the corresponding author} ; Bo-hui YANG^a ; ^{yangbh2004@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chinese fine-wool sheep ; indigenous sheep breeds ; genetic relationship ; gene flow ; microsatellites
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：15
• 期：4
• 页码：862-871
• 全文大小：2025 K

文摘

The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array. Meanwhile, genetic variations among 482 individuals from 9 populations were genotyped with 10 microsatellites. In this study, we found high genetic polymorphisms for the microsatellites, while 7 loci in the Chinese superfine Merino strain (Xinjiang types) (CMS) and 5 loci in Gansu alpine superfine-wool sheep strain (GSS) groups were found deviated from Hardy-Weinberg equilibrium (HWE). Genetic drift F_ST=0.019 (P<0.001) and high gene flows were detected in all the 7 fine-wool sheep populations. Phylogenetic analysis showed fine-wool sheep populations were clustered in a group independent from the Chinese indigenous breeds such that the 7 fine-wool sheep clustered distinct from Liangshan semifine-wool sheep (LS) and Hu sheep (HY) reflected by different population differentiation analyses. Overall, our findings suggested that all fine-wool sheep populations have close genetic relationship, which is consistent with their breeding progress. These populations, therefore, can be regarded as open-breeding populations with high levels of gene flows. Furthermore, the two superfine-wool strains, viz., CMS and GSS, might be formed by strong artificial selection and with frequent introduction of Australian Merino. Our results can assist in breeding of superfine-wool sheep and provide guidance for the cultivation of new fine-wool sheep breeds with different breeding objectives.