Effective remediation of aged HMW-PAHs polluted agricultural soil by the combination of Fusarium sp. and smooth bromegrass (Bromus inermis Leyss.)

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• 作者：Wei SHI^a ; ^{649985324@qq.com} ; Xue-na ZHANG^a ; Hai-bin JIA^a ; Sheng-dong FENG^a ; Zhi-xin YANG^a ; ^{yangzhixin@126.com} ; Ou-ya ZHAO^a ; Yu-ling LI^b
• 关键词：coal mining area ; HMW-PAHs ; Fusarium ; smooth bromegrass ; enzymatic activities
• 刊名：Journal of Integrative Agriculture
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：16
• 期：1
• 页码：199-209
• 全文大小：470 K
• 卷排序：16

文摘

Fusarium sp. strain ZH-H2 is capable to degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs), smooth bromegrass (Bromus inermis Leyss.) can also degrade 4- to 6-ring PAHs. Pot experiments were conducted to investigate how bromegrass and different inoculum sizes of ZH-H2 clean up HMW-PAHs in agricultural soil derived from a coal mine area. The results showed that, compared with control, different sizes of inocula of ZH-H2 effectively degraded HMW-PAHs, with removal rates of 19.01, 34.25 and 29.26% for 4-, 5- and 6-ring PAHs in the treatment with 1.0 g kg−1 ZH-H2 incubation after 90 d. After 5 mon of cultivation, bromegrass reached degradation rate of these compounds by 12.66, 36.26 and 36.24%, respectively. By adding strain ZH-H2 to bromegrass, HMW-PAHs degradation was further improved up to 4.24 times greater than bromegrass (W), in addition to the degradation rate of Bbf decrease. For removal rates of both 5- and 6-ring PAHs, addition of 0.5 g kg−1Fusarium ZH-H2 to pots with bromegrass performed better than addition of 0.1 g kg−1, while the highest concentration of 1.0 g kg−1Fusarium ZH-H2 did not further improve degradation. Degradation of 4-ring PAHs showed no significant difference among different ZH-H2 incubations with bromegrass treatments. We found that the degradation rates of 4-, 5- and 6-ring PAHs in all treatments are significantly correlated in a positive, linear manner with activity of lignin peroxidase (LiP) (r=0.8065, 0.9350 and 0.9165, respectively), while degradation of 5- and 6-ring PAHs is correlated to polyphenoloxidase (PPO) activity (r=0.7577 and 07806). Our findings suggest that the combination of Fusarium sp. ZH-H2 and bromegrass offers a suitable alternative for phytoremediation of aged PAH-contaminated soil in coal mining areas, with a recommended inoculation size of 0.5 g Fusarium sp. ZH-H2 per kg soil.