Rapamycin together with TGF-尾1, IL-2 and IL-15 induces the generation of functional regulatory 纬未T cells from human peripheral blood mononuclear cells

详细信息    查看全文

• 作者：Yanjun Gu ; Yongxian Hu ; Kaimin Hu ; Weichao Liao ; Feilin Zheng ; Xiaohong Yu ; He Huang
• 关键词：纬未Treg ; Rapamycin ; Induction ; Foxp3 ; Immunosuppression
• 刊名：Journal of Immunological Methods
• 出版年：15 January, 2014
• 年：2014
• 卷：402
• 期：1-2
• 页码：82-87
• 全文大小：879 K

文摘

Regulatory 纬未T cell (纬未Treg) is a recently reported subset of 纬未T cells characterized by both expressions of TCR纬未 and Foxp3, with potential immunosuppressive functions. However, the further studies of 纬未Treg are limited mainly due to its low quantities in vivo and the lack of methods to induce 纬未Treg largely in vitro. Here we show that rapamycin together with TGF-尾1, IL-2 and IL-15 can induce and expand 纬未Tregs derived from human peripheral blood mononuclear cells efficiently in vitro. Thus-induced 纬未Tregs express high levels of Foxp3 and CD25, and display significant immunosuppressive activities in vitro.