MMP20 and ARMS2/HTRA1 Are Associated with Neovascular Lesion Size in Age-Related Macular Degeneration

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• 作者：Yumiko Akagi-Kurashige ; MD¹ ; ² ; Kenji Yamashiro ; MD ; PhD¹ ; ^{yamashro@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Norimoto Gotoh ; MD ; PhD¹ ; ² ; Masahiro Miyake ; MD¹ ; ² ; Satoshi Morooka ; MD¹ ; Munemitsu Yoshikawa ; MD¹ ; ² ; Isao Nakata ; MD ; PhD¹ ; ² ; Kyoko Kumagai ; MD ; PhD¹ ; Akitaka Tsujikawa ; MD ; PhD¹ ; Ryo Yamada ; MD ; PhD² ; Fumihiko Matsuda ; PhD² ; Masaaki Saito ; MD ; PhD³ ; Tomohiro Iida ; MD ; PhD⁴ ; Masako Sugahara ; MD⁵ ; Yasuo Kurimoto ; MD ; PhD⁵ ; Ching-Yu Cheng ; MD ; PhD⁶ ; ⁷ ; ⁸ ; ⁹ ; Chiea-Chuen Khor ; MBBS ; PhD⁶ ; ¹⁰ ; ¹¹ ; Tien-Yin Wong ; FRCS ; PhD⁶ ; ⁷ ; ⁸ ; ⁹ ; Nagahisa Yoshimura ; MD ; PhD¹ ; on behalf of the Nagahama Cohort Research Group^&lowast ; ; Takeo Nakayama ; Akihiro Sekine ; Shinji Kosugi ; Takahisa Kawaguchi ; Ryo Yamada ; Yasuharu Tabara ; Fumihiko Matsuda
• 关键词：AMD ; age-related macular degeneration ; cDNA ; complementary DNA ; CNV ; choroidal neovascularization ; GLD ; greatest linear dimension ; GWAS ; genome-wide association study ; LD ; linkage disequilibrium ; MMP ; matrix metalloprotease ; PCV ; polypoidal choroidal vasculopathy ; RPE ; retinal pigment epithelium ; SNP ; single nucleotide polymorphism ; tAMD ; typical age-related macular degeneration ; VEGF ; vascular endothelial growth factor
• 刊名：Ophthalmology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：122
• 期：11
• 页码：2295-2302.e2
• 全文大小：934 K

文摘

Age-related macular degeneration (AMD) is the leading cause of severe visual impairment. Despite treatment, a central scotoma often remains. The size of the scotoma depends on the lesion size of the choroidal neovascular membrane and significantly affects the patient's quality of life, and the lesion size of neovascularization also affects response to treatments. The aim of this study was to identify genes associated with the neovascular lesion size in neovascular AMD.

Design

A genome-wide association study (GWAS).

Participants

We included 1146 Japanese patients with neovascular AMD.

Methods

We performed a 2-stage GWAS for the lesion size of AMD as a quantitative trait among 1146 (first stage: 727, second stage: 419) Japanese patients with neovascular AMD. Lesion size was determined by the greatest linear dimension measured with fluorescein angiography examination before treatment. We examined the association between the genotypic distribution of each single nucleotide polymorphism (SNP) and the trait using an additive model adjusted for age and sex. To evaluate the associations between AMD development and SNPs associated with lesion size, we also performed a case-control study by using the genotype data from these 1146 Japanese patients as case subjects and the fixed dataset from the Nagahama Study as control subjects.

Main Outcome Measures

Genes associated with the lesion size in neovascular AMD.

Results

In the discovery stage, rs10895322 in MMP20 showed a genome-wide significant P value of 6.95×10⁻⁸, and rs2284665 in ARMS2/HTRA1 showed a P value of 1.55×10⁻⁷. The associations of these 2 SNPs were successfully replicated in the replication stage, and a meta-analysis of both stages showed genome-wide significant P values (2.80×10⁻⁹ and 4.41×10⁻⁹, respectively). In a case-control study using 3248 Japanese subjects as controls, we could not find contribution of MMP20 rs10895322 for AMD development. Although MMP20 has been thought to be expressed only in dental tissues, we confirmed MMP20 expression in the human retina and retinal pigment epithelium/choroid with polymerase chain reaction.

Conclusions

The growth of choroidal neovascularization in AMD would be affected by 2 genes: MMP20, a newly confirmed gene expressed in the retina, and ARMS2/HTRA1, a well-known susceptibility gene for AMD.