Cucurbitacin D isolated from Trichosanthes kirilowii
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- 作者：Norito Takahashi ; Yasuhiro Yoshida ; Tsutomu Sugiura ; Koji Matsuno ; Akihiro Fujino ; Uki Yamashita
- 关键词：Cucurbitacin D ; Trichosanthes kirilowii ; Hepatocellular carcinoma ; Apoptosis ; Caspase-3 ; JNK
- 刊名：International Immunopharmacology
- 出版年：2009
- 出版时间：April 2009
- 年：2009
- 卷：9
- 期：4
- 页码：508-513
- 全文大小：893 K
文摘
The aim of the present study is to examine the effects of the anti-tumor component isolated from Trichosanthes kirilowii on human hepatocellular carcinoma cells. Using Sephadex G-25 column chromatography, Sep-Pak Plus C18 cartridge and high-performance liquid chromatography (HPLC), we isolated the active component from trichosanthes extract. By fast atom bombardment mass spectrometric analysis, the molecular mass of the active fraction was determined, the active components identified, and their mechanisms of action were analyzed by cell growth assay, cell cycle analysis, TUNEL staining and Western blot analysis. We found that the anti-tumor components isolated from the extract of trichosanthes (EOT) are cucurbitacin D and dihydrocucurbitacin D, and suggest that cucurbitacin D induces apoptosis through caspase-3 and phosphorylation of JNK in hepatocellular carcinoma cells. These results suggest that cucurbitacin D isolated from Trichosanthes kirilowii could be a valuable candidate for anti-tumor drug.