Functional and morphological alterations of the urinary bladder in type 2 diabetic FVB^db/db mice

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• 作者：Liyang Wu^a ; ^b ; Xiaodong Zhang^b ; Nan Xiao^a ; ^c ; Yexiang Huang^a ; ^d ; Michael Kavran^e ; Rania A. Elrashidy^a ; ^f ; Mingshuai Wang^a ; ^b ; Firouz Daneshgari^a ; Guiming Liu^a ; ^{guiming.liu@case.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bladder ; Type 2 diabetes ; FVBdb/db mice ; Obesity ; Detrusor overactivity
• 刊名：Journal of Diabetes and its Complications
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：30
• 期：5
• 页码：778-785
• 全文大小：1286 K

文摘

Diabetic bladder dysfunction (DBD) has been extensively studied in animal models of type 1 diabetes. We aimed to examine the functional and morphological alterations of the urinary bladder in a type 2 diabetes model, FVB^db/db mice.

Methods

FVB^db/db mice and age-matched FVB/NJ control mice were tested at either 12, 24 or 52 weeks of age. Body weight, blood glucose and glycated hemoglobin (HbA1c) levels were measured. Bladder function was assessed by measurement of 24-h urination behavior and conscious cystometry. Bladder was harvested for Masson's Trichrome staining and morphometric analysis.

Results

The body weights of FVB^db/db mice were twice as those of FVB/NJ control mice. The blood glucose and HbA1c levels were higher in FVB^db/db mice at 12 and 24 weeks, but not at 52 weeks. A significant increase in the mean volume per void, but decrease in the voiding frequency, in FVB^db/db mice was observed. Cystometry evaluation showed increased bladder capacity, voided volume, and peak micturition pressure in FVB^db/db mice compared with FVB/NJ mice. Morphometric analysis revealed a significant increase in the areas of detrusor muscle and urothelium in FVB^db/db mice. In addition, some FVB^db/db mice, especially males at 12 and 24 weeks, showed small-volume voiding during 24-h urination behavior measurement, and detrusor overactivity in the cystometry measurement.

Conclusions

The FVB^db/db mouse, displaying DBD characterized by not only increased bladder capacity, void volume, and micturition pressure, but also bladder overactivity, is a useful model to further investigate the mechanisms of type 2 diabetes-related bladder dysfunction.