Salicylic acid retention impairs aspirin reactivity in type 2 diabetes

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• 作者：Haowen Zhang^a ; ^b ; ¹ ; Hao Xie^c ; ¹ ; Xiao Zheng^c ; ^d ; Yingying Chai^c ; Zhiyuan Tang^c ; Hanyu Chen^a ; ^b ; Feiyan Li^c ; Heier Christoph^e ; Jiandong Chen^b ; Weixin Sun^a ; ^b ; Hui Ye^c ; Shiguang Wang^a ; ^b ; Haiping Hao^c ; ^{hhp_770505@hotmail.com} ; Xiaohu Chen^b ; ^{chenxhdoctor@126.com}
• 关键词：HAPR ; Diabetes ; Salicylic acid ; Cyclooxygenase-2 ; Platelet
• 刊名：European Journal of Pharmacology
• 出版年：2017
• 出版时间：5 January 2017
• 年：2017
• 卷：794
• 期：Complete
• 页码：234-245
• 全文大小：1804 K
• 卷排序：794

文摘

High on-aspirin platelet reactivity (HAPR) has been associated with compromised aspirin efficacy in patients with diabetes suffering from acute cardiovascular events, but the key mechanisms remain elusive. The objective of this study was to uncover the potential link between pathogenic accumulation of salicylic acid (SA), the major metabolite of aspirin, and HAPR in diabetic state. Aspirin failed to inhibit platelet CD62P expression and thromboxane (TX) B2/6-keto-prostaglandin（PG）F1α ratio in a type 2 diabetes mellitus (T2DM) mice model, particularly in the female, which were unanimously accompanied by significantly higher plasma SA concentrations. Pre-administration with SA increased both platelet CD62P expression and TXB2/6-keto-PGF1α ratio in female T2DM mice, while pretreatment with NaHCO3 caused the opposite effect. On the in vitro human umbilical vein endothelial cells (HUVECs)-platelet interaction assay, SA suppressed inflammation-induced cyclooxygenase-2 upregulation on HUVECs and attenuated their inhibitory effect on platelet aggregation in a dose-dependent manner. The prolonged retention of SA in diabetes may be partially explained by the downregulation of various SA efflux transporters in the kidney and the decreased urine pH. Importantly, in female aspirin non-responsive patients, the trough plasma concentration of SA are markedly increased with T2DM treated with long-term aspirin, and TXB2/6-keto-PGF1α ratio and uric acid level in plasma are positively correlated with SA concentration. Our findings support that the accumulation of SA represents an important factor in causing HAPR in diabetes, and that targeting impaired SA excretion may become a novel intervention strategy to diabetes-associated HAPR.