Assessment of the Campylobacter jejuni and C. coli in broiler chicken ceca by conventional culture and loop-mediated isothermal amplification method

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• 作者：Islam Ibrahim Sabike^a ; ^d ; ^{Islam.sabek@fvtm.bu.edu.eg} ; Ryoko Uemura^a ; ^b ; ^{uemurary@cc.miyazaki-u.ac.jp} ; Yumi Kirino^b ; ^{kirinoyumi@cc.miyazaki-u.ac.jp} ; Hirohisa Mekata^b ; ^c ; ^{mekata@cc.miyazaki-u.ac.jp} ; Satoshi Sekiguchi^a ; ^b ; ^{sekiguchi@cc.miyazaki-u.ac.jp} ; Ayman Samir Farid^e ; ^{aymansf@yahoo.com} ; Yoshitaka Goto^a ; ^b ; ^{a0d502u@cc.miyazaki-u.ac.jp} ; Yoichiro Horii^a ; ^b ; ^{horii@cc.miyazaki-u.ac.jp} ; Wataru Yamazaki^a ; ^b ; ^{yamazakiw@cc.miyazaki-u.ac.jp}
• 关键词：Food poisoning ; Prevalence ; Campylobacter ; Seasonal change ; Broiler chicken ceca ; LAMP
• 刊名：Food Control
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：74
• 期：Complete
• 页码：107-111
• 全文大小：239 K
• 卷排序：74

文摘

Campylobacter loads range between 6.4 and 9.0 log CFU/g in Japanese broiler ceca. A LAMP assay directly detects C. jejuni/C. coli in broiler ceca within 90 min. Sensitivity and specificity for LAMP were equal to conventional culture methods. Prevalence in the broiler flocks was correlated with the food poisoning frequency. High broiler prevalence is a crucial risk factor in food poisoning in humans.