Administration of growth hormone modulates the gene expression of basic fibroblast growth factor in rat costal cartilage, both in vivo and in vitro
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- 作者：Izumi ; Toshihiro ; Shida ; Jun-ichi ; Jingushi ; Seiya ; Hotokebuchi ; Takao ; Sugioka ; Yoichi
- 关键词：Growth hormone ; Basic fibroblast growth factor ; Cartilage
- 刊名：Molecular and Cellular Endocrinology
- 出版年：1995
- 出版时间：July, 1995
- 年：1995
- 卷：112
- 期：1
- 页码：95-99
- 全文大小：442 K
文摘
We examined the effect of growth hormone on local growth factor mRNA expression in male Sprague-Dawley rats. Repetitive systemic administration of growth hormone (0.4 IU every 4 h) increased the expression of IGF-I mRNA up to 2.8-fold in costal cartilage tissue compared with controls. Basic FGF (bFGF) mRNA expression gradually increased up to 15.5-fold compared with pre-injection samples, where the mRNA expression was 5.3-times greater than vehicle-injected controls. TGF-β mRNA showed little changes. Moreover, one μg/ml of growth hormone enhanced the expression of bFGF mRNA in costal chondrocytes in culture. We conclude that growth hormone increased the local expression of bFGF, as well as that of IGF-I, in cartilage, and suggest that bFGF is directly regulated by growth hormone within a local area.