Expression of the c-fos-monomeric red fluorescent protein 1 fusion gene in the spinal cord and the hypothalamic paraventricular nucleus in transgenic rats after nociceptive stimulation

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• 作者：Toru Ishikura ; Hitoshi Suzuki ; Mitsuhiro Yoshimura ; Jun-ichi Ohkubo ; Akiko Katoh ; Toyoaki Ohbuchi ; Motoko Ohno ; Hiroaki Fujihara ; Makoto Kawasaki ; Hideo Ohnishi ; Toshitaka Nakamura ; Yoichi Ueta
• 关键词：AVP ; arginine vasopressin ; CRH ; corticotropin-releasing hormone ; eGFP ; enhanced green fluorescent protein ; Fos-LI ; Fos-like immunoreactivity ; mRFP1 ; monomeric red fluorescent protein 1 ; PB ; phosphate buffer ; PBS ; phosphate buffer saline ; PVN ; paraventricu
• 刊名：Brain Research
• 出版年：2012
• 出版时间：15 October, 2012
• 年：2012
• 卷：1479
• 期：Complete
• 页码：52-61
• 全文大小：1424 K

文摘

We generated transgenic rats expressing the c-fos and monomeric red fluorescent protein 1 (mRFP1) fusion gene in the central nervous system after adequate stimulation. In the present study, the time-course of the induction patterns of mRFP1 fluorescence in the spinal cord and the paraventricular nucleus (PVN) was compared with that of Fos-like immunoreactivity (LI) within 24 h after subcutaneous (s.c.) injection of 0.9 % saline and 5 % formalin in both hind paws. Control rats were not treated. In the control and saline/formalin injected rats, scattered mRFP1 fluorescence in the spinal cord and the PVN was observed at 0 min, though there was little Fos-LI in the same region. The mRFP1 fluorescence in the spinal cord and the PVN was increased at 3 h after formalin. On the other hand, the changes of Fos-LI in the spinal cord and the PVN were relatively shorter than those of the mRFP1 fluorescence after formalin. These results suggest that the c-fos-mRFP1 fusion gene expression is slightly upregulated in normal conditions and nociceptive stimulation-induced induction of the fusion gene may be maintained longer than the endogenous c-fos gene expression in the spinal cord and the PVN. Next, nocifensive behavior and mRFP1 fluorescence and Fos-LI in the spinal cord and the PVN after s.c. injection of formalin, 4¦Á-phorbol 12,13-didecanoate (4¦Á-PDD) and saline were compared. Although the 4¦Á-PDD injected rats seldom displayed nocifensive behaviors like s.c. saline injection, 4¦Á-PDD injection caused mRFP1 fluorescence and Fos-LI significantly in the spinal cord and the PVN unlike s.c. saline injection.