Quantitative estimation of the replication kinetics of genotype 2 PRRSV strains with different levels of virulence in vitro
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- 作者：Jianguo Donga ; b ; 1 ; Gang Wanga ; 1 ; Yonggang Liua ; Wenda Shia ; Jianan Wua ; Huiqiang Wena ; Shujie Wanga ; Zhijun Tiana ; Xuehui Caia ; cai139@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：PRRSV ; RT-qPCR ; Replication kinetics ; Viral loads
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：234
- 期：Complete
- 页码：87-89
- 全文大小：516 K
文摘
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A sensitive, specific, and reproducible PRRSV RT-qPCR assay was established.
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First report the replication kinetics of LP- and HP-PRRSV strain in MARC-145 cells and PAMs.
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HP-PRRSV (Hu-N4) replicated to a higher titer than LP-PRRSV (CH-1a) in vitro.