Immobilization of SMG1-F278N lipase onto a novel epoxy resin: Characterization and its application in synthesis of partial glycerides
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- 作者：Xingxing Lia ; 1 ; Daoming Lia ; 1 ; Weifei Wangb ; Rabia Durranic ; Bo Yangc ; Yonghua Wang ; Ph.D.a ; yonghw@scut.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Immobilization ; Mono- and diacylglycerol lipase ; Characterization ; Partial glycerides ; Reusability
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：133
- 期：Complete
- 页码：154-160
- 全文大小：1373 K
文摘
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SMG1-F278N was covalently immobilized onto a novel epoxy resin ECR8285.
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The immobilized SMG1-F278N improved their performance in wider ranges of pH and temperature.
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Thermostability of SMG1-F278N was significantly improved after immobilization.
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High yield (79%) of partial glycerides was obtained by immobilized SMG1-F278N-catalyzed esterification of glycerol and oleic acids.
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The immobilized SMG1-F278N kept 98% of its initial activity after being used for 7 cycles.