A novel fluorescence immunoassay for the sensitive detection of Escherichia coli O157:H7 in milk based on catalase-mediated fluorescence quenching of CdTe quantum dots
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- 作者：Rui Chena ; b ; Xiaolin Huangb ; Juan Lib ; Shan Shanb ; Weihua Laib ; Yonghua Xionga ; b ; yhxiongchen@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Fluorescence immunoassay ; Detection ; Catalase ; Escherichia coli O157 ; H7 ; Milk
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：1 December 2016
- 年：2016
- 卷：947
- 期：Complete
- 页码：50-57
- 全文大小：1106 K
文摘
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A novel fluorescence immunoassay was developed for the ultrasensitive detection of E. coli O157:H7.
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This detection was achieved through the combination of the high bioactivity of CAT and H2O2-sensitive QDs.
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The activity of CAT to H2O2 is 1000 folds higher than that of the HRP to tetramethylbenzidine.
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The limit of detection of the proposed method could reach as low as 102 CFU/mL.