Construction of genetic linkage maps of guppy (Poecilia reticulata) based on AFLP and microsatellite DNA markers

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• 作者：Xueyan Shen ; Guanpin Yang ; Yongjian Liu ; Meijie Liao ; Xiaochen Wang ; Mingzhuang Zhu ; Weibo Song ; Guiwei Zou ; Qiwei Wei ; Dengqiang Wang ; Daqing Chen
• 关键词：Poecilia reticulata ; Linkage map ; AFLP ; Microsatellite DNA
• 刊名：Aquaculture
• 出版年：2007
• 出版时间：3 October 2007
• 年：2007
• 卷：271
• 期：1-4
• 页码：178-187
• 全文大小：1005 K

文摘

Genetic linkage maps of guppy (Poecilia reticulata) were constructed using microsatellite DNA and AFLP markers and a pseudo-testcross mapping strategy. (AC)_n microsatellite DNA markers were developed and used to genotype sixty-one full-sib progenies (F₁) and their two parents. Of 293 microsatellite DNA markers, 101 segregated at 1:1 or 1:1:1:1 ratios. In addition, 336 AFLP markers segregated also in F₁ progenies at 1:1 ratio, which were produced using 91 primer combinations. All these markers were mapped with two linkage maps produced, one each parent. Female map included 135 markers in 22 linkage groups, covering a total of 1267.7 cM in length. Male map included 172 markers in 20 linkage groups, covering a total of 1771.2 cM in length.