Acylglycerol kinase functions as an oncogene and an unfavorable prognostic marker of human gliomas

详细信息    查看全文

• 作者：Nan Liu ; MS^a ; Zhen Wang ; MS^a ; Yingduan Cheng ; PhD^b ; Pengxing Zhang ; MS^a ; Xin Wang ; PhD^c ; Hongwei Yang ; PhD^c ; Hui Liu ; MS^a ; Yongsheng Zhang ; MD^d ; ^{Zhangys_td@163.com} ; Yanyang Tu ; MD^a ; ^c ; ^{tu.fmmu@gmail.com}
• 关键词：Acylglycerol kinase ; Glioma ; Prognosis ; Cell proliferation ; Migration ; Invasion
• 刊名：Human Pathology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：58
• 期：Complete
• 页码：105-112
• 全文大小：1322 K
• 卷排序：58

文摘

Acylglycerol kinase (AGK) regulates various cellular processes involved into tumorigenesis and tumor progression. To investigate involvement of AGK in human gliomas, here, quantitative real-time polymerase chain reaction, Western blot, and immunohistochemistry analyses were performed to respectively detect the expression of AGK mRNA and protein in glioma and nonneoplastic brain tissue specimens. Then, the associations of AGK expression with various clinicopathological characteristics and patients' prognosis were statistically evaluated. Moreover, the effects of siRNA-mediated AGK knockdown on glioma cell proliferation, migration, and invasion were respectively assessed via Cell Counting Kit-8 and Transwell assays in vitro. As a result, AGK expression, at both mRNA and protein levels, were markedly up-regulated in glioma tissues compared with nonneoplastic brain tissues (both P < .001). In addition, high AGK expression was significantly associated with the grade of malignancy and poor prognosis in glioma patients (all P < .05). Importantly, Cox regression model of multivariate analysis identified AGK expression as an independent prognostic factor for glioma patients (P = .03). Furthermore, silencing the expression of AGK dramatically suppressed cell proliferation, migration, and invasion of glioma cells in vitro (all P < .05). In conclusion, AGK up-regulation may be involved into glioma development and progression, highlighting its prognostic value for the treatment of patients with this malignancy. Further loss-of-function experiments suggest that AGK might play an important role in the viability and motility of glioma cells, implying its potentials as an attractive therapeutic target for this tumor.