An isothermal, label-free, and rapid one-step RNA amplification/detection assay for diagnosis of respiratory viral infections

详细信息    查看全文

• 作者：Bonhan Koo<sup>asup> ; Choong Eun Jin<sup>asup> ; Tae Yoon Lee<sup>bsup> ; Jeong Hoon Lee<sup>csup> ; Mi Kyoung Park<sup>dsup> ; Heungsup Sung<sup>esup> ; Se Yoon Park<sup>fsup><sup> ; sup><sup>gsup> ; Hyun Jung Lee<sup>fsup> ; Sun Mi Kim<sup>fsup> ; Ji Yeun Kim<sup>fsup> ; Sung-Han Kim<sup>fsup><sup> ; sup><sup>kimsunghanmd@hotmail.comsup> ; Yong Shin<sup>asup><sup> ; sup><sup>shinyongno1@gmail.comsup>
• 关键词：Respiratory disease ; Emerging infectious disease ; RNA amplification/detection ; Label-free biosensor
• 刊名：Biosensors and Bioelectronics
• 出版年：2017
• 出版时间：15 April 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：187-194
• 全文大小：856 K
• 卷排序：90

文摘

Employed isothermal amplification with bio-photonic sensor for detection of viral RNAs. Rapid RNA amplification and detection system simultaneously within 20 min. Validated the utility of the system in human respiratory viral samples. Versatile technology can be readily applied to other RNA-based studies and diseases.