Increasing the miR-126 expression in the peripheral blood of patients with diabetic foot ulcers treated with maggot debridement therapy

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• 作者：Jie Zhang¹ ; ^{xiaopengyou1226@sina.com} ; Xin-Juan Sun¹ ; ^{27966509@qq.com} ; Jin'an Chen ^{cysc111@163.com} ; Zhi Wei Hu ^{huzhiwei_yu1208@163.com} ; Lei Wang ^{872819805@qq.com} ; Dong Mei Gu ; Ai-ping Wang ; ^{wap454hospital@hotmail.com}
• 关键词：Maggot debridement therapy ; miR-126 ; Diabetic foot ulcer ; miRNA ; Expression
• 刊名：Journal of Diabetes and its Complications
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：31
• 期：1
• 页码：241-244
• 全文大小：327 K
• 卷排序：31

文摘

miR-126 may increase angiogenesis in patients with diabetic foot ulcers (DFUs) treated with maggot debridement therapy (MDT).MethodsReal-time quantitative PCR was used to detect expression of miR-126 mRNA in the peripheral blood among the non-diabetic population, type 2 diabetes mellitus patients without DFU, and patients with DFUs of type 2 diabetes mellitus. The expression of miR-126 mRNA in the peripheral blood of patients with DFUs was observed before and after MDT. Finally, human umbilical vein endothelial cells (HUVEC) were utilized to explore miR-126 mRNA expression with maggot excretions/secretions (ES).ResultsIn the patients with DFUs, the miR-126 mRNA expression level in the peripheral blood was less than that type 2 diabetes mellitus patients without DFU, and much lower than that in the non-diabetic population (P < 0.001). The miR-126 expression level was significantly increased in those DFU patients treated with MDT (P < 0.05). Finally, using HUVEC co-cultured with ES, we showed the ES increased miR-126 expression in vitro (P < 0.001).ConclusionMDT upregulates the miR-126 expression in the peripheral blood of patients with DFUs.