Differences in TLR9-dependent inhibitory effects of H₂O₂-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species

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• 作者：Yukihiro Hiramatsu ; Tomomitsu Satho ; ^{satho@cis.fukuoka-u.ac.jp} ; Keiichi Irie ; Shota Shiimura ; Takahiro Okuno ; Tanjina Sharmin ; Saori Uyeda ; Yuki Fukumitsu ; Yukihiko Nakashima ; Fumio Miake ; Nobuhiro Kashige
• 关键词：Lactic acid bacteria ; Genomic DNA ; Anti-inflammatory effect ; NF-¦ÊB ; Toll-like receptor 9
• 刊名：Microbes and Infection
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：15
• 期：2
• 页码：96-104
• 全文大小：818 K

文摘

Lactic acid bacteria (LAB) show anti-inflammatory effects, and their genomic DNA was identified as one of the anti-inflammatory components. Despite the differences in anti-inflammatory effects between live LAB dependent not only on genus but also species, this effect has not been compared at the genomic DNA level. We compared the anti-inflammatory effects of the genomic DNA from five Lactobacillus species¡ªLactobacillus acidophilus, Lactobacillus casei, Lactobacillus gasseri, Lactobacillus plantarum, and Lactobacillus reuteri¡ªusing Caco-2 cells. To evaluate anti-inflammatory effects, decreases in H₂O₂-induced IL-8 secretion and inhibition of H₂O₂-induced NF-¦ÊB/I¦ÊB-¦Á system activation were examined. All LAB genomic DNAs dose-dependently decreased H₂O₂-induced IL-8 secretion and inhibited H₂O₂-induced NF-¦ÊB/I¦ÊB-¦Á system activation. Comparison of these effects between Lactobacillus species showed that the anti-inflammatory effects of L. acidophilus genomic DNA are lower than those of the other species. Furthermore, suppression of Toll-like receptor 9 (TLR9), a specific receptor of bacterial DNA, expression by RNAi abolished the decrease of H₂O₂-induced IL-8 secretion and inhibition of H₂O₂-induced NF-¦ÊB/I¦ÊB-¦Á system activation by LAB genomic DNA. Our results demonstrated that the anti-inflammatory effects of genomic DNA differ between Lactobacillus species and TLR9 is one of the major pathways responsible for the anti-inflammatory effect of LAB genomic DNA.