Electrochemical immunosensor for N6-methyladenosine RNA modification detection
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- 作者：Huanshun Yina ; b ; 1Author Vitae ; Yunlei Zhoua ; 1Author Vitae ; Zhiqing YangaAuthor Vitae ; Yunlong GuoaAuthor Vitae ; Xinxu WangaAuthor Vitae ; Shiyun Aia ; ashy@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Xiansheng Zhangb ; zhangxs@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：N6-methyladenosine ; RNA methylation ; Immunosensor ; Cytokinin ; Anti-N6-methyladenosine antibody ; mRNA
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2015
- 出版时间：31 December 2015
- 年：2015
- 卷：221
- 期：Complete
- 页码：1-6
- 全文大小：1243 K
文摘
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An electrochemical immunosensor was fabricated for RNA methylation detection.
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N6-methyladenosine antibody was used as capture probe for RNA methylation.
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The developed method showed wide linear range from 0.01 to 10 nM and the detection limit was 2.57 pM.
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The immunosensor was applied to detect the methylation level of mRNA in rice seedling leaves.