Optical readout of a nanoparticle based sensor by cavity ring-down spectroscopy

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• 作者：T. Zeuner¹ ; ^{thomas.zeuner@ipht-jena.de" class="auth_mail}Author Vitae ; W. Paa¹Author Vitae ; G. Schmidl¹Author Vitae ; D. ZopfAuthor Vitae ; T. HenkelAuthor Vitae ; A. Csá ; kiAuthor Vitae ; W. FritzscheAuthor Vitae
• 关键词：Cavity ring-down ; Plasmonic nanoparticles sensor ; DNA-sensing
• 刊名：Sensors and Actuators B: Chemical
• 出版年：May, 2014
• 年：2014
• 卷：195
• 期：Complete
• 页码：352-358
• 全文大小：1542 K

文摘

We combine the plasmonic properties of metallic nanoparticles enabling a label-free bioanalytical approach with the cavity ring-down method (CRD) in a miniaturized fluidic device as a sensitive and fast optical photon lifetime measurement. Nanoparticles (Ag triangles and Au nanodots) are immobilized within a specially designed microfluidic chip (MFC) that is situated inside an optical cavity. Our approach allows to measure absorption/loss changes of 0.02% - well below the typical detection limit (<1%) of conventional spectrometers. The proof of principle is demonstrated by sensing refractive index changes of the solution inside the chip. Furthermore, a first bioanalytical application example is presented. Therefore, the binding of 2 脳 10⁸ DNA sequences from the phytopathogene microorganism Phytophthora kernoviae onto nanoparticles functionalized with complementary DNA molecules could be detected.