A two-photon fluorescent probe for imaging hydrogen sulfide in living cells

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• 作者：Tianyu Liu ; Xinfu Zhang ; Qinglong Qiao ; Chunyan Zou ; Lei Feng ; Jingnan Cui ; Zhaochao Xu
• 关键词：Two-photon fluorescence microscopy ; Fluorescent probes ; Hydrogen sulfide ; Thiolysis ; High selectivity ; Cells imaging
• 刊名：Dyes and Pigments
• 出版年：December, 2013
• 年：2013
• 卷：99
• 期：3
• 页码：537-542
• 全文大小：909 K

文摘

Fluorescent probes for hydrogen sulfide have received considerable attention because of the biological significance of H₂S recognized recently. Two-photo microscopy offers advantages of increased penetration depth, localized excitation, and prolonged observation time. However, two-photon fluorescent probes for H₂S are still rare. In this work, we introduced a dinitrophenyl ether group into the 4-position of 1,8-naphthalimide, which acts as the H₂S reactive site, to efficiently yield compound NI-NHS as a two-photo fluorescent probe for H₂S. The probe NI-NHS has a high selectivity for H₂S over competitive anions and sulfide-containing analytes. This probe exhibits turn-on fluorescence detection of H₂S in bovine serum and two-photon fluorescent imaging of H₂S in living cells.