Overexpression of lycopene ε-cyclase gene from lycium chinense confers tolerance to chilling stress in Arabidopsis thaliana
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- 作者：Xinyu Songa ; b ; Jinjin Diaoa ; Jing Jib ; jijing@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Gang Wangb ; ; Zhaodi Lia ; Jiang Wua ; c ; Tchouopou Lontchi Josineb ; Yurong Wangd
- 关键词：ROS ; reactive oxygen species ; ORF ; open reading frame ; SOD ; superoxide dismutase ; POD ; peroxidase ; MDA ; malondialdehyde ; PSII ; photosystem II ; NPQ ; Non-photochemical quenching ; OD ; optical density ; IPTG ; isopropyl-β-D-thiogalactoside ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; HPLC ; high performance liquid chromatography ; PVDF ; polyvinylidene fluoride ; cDNA ; complementary DNA ; LHCII ; light harvest complex II
- 刊名：Gene
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：576
- 期：1
- 页码：395-403
- 全文大小：910 K
文摘
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We cloned the Lcε-LYC gene from carotenoids-rich plant Lycium chinense, and analyzed its characteristics from multiple perspectives.
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We transformed this novel Lcε-LYC gene into A. thaliana, and found that the transgenics had improved tolerance to chilling stress.
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The increased chilling tolerance in transgenic plants was ascribed to the enhanced lutein content and its roles in chlorophyll antenna size of photosystem II, photoprotection and membrane stabilization.